By Lita A. Freeman (auth.), Lita A. Freeman (eds.)
The lipid-rich and differently hard nature of many key tissues complicates many facets of present learn, and purposes of the original nature of lipoproteins and their organic results has engendered specific and very important methodologies. In Lipoproteins and heart problems: tools and Protocols, specialists within the box current a compendium of complex and classical molecular biology equipment distinctive in the direction of lipoprotein, atherosclerosis, and vascular biology learn, bringing jointly in one quantity an up to date set of protocols and methods for ways now riding the latest advances, in addition to classical equipment which are nonetheless ordinary. one of many subject matters lined during this state of the art paintings, the ebook delves into an important ideas akin to quantitative real-time PCR, microarrays, RT-PCR laser catch microdissection, and tissue-specific gene overexpression, knockout, and knockdown methodologies, together with AAV as a liver-directed gene supply automobile. Written within the hugely profitable Methods in Molecular Biology™ sequence structure, chapters contain introductions to their respective matters, lists of the mandatory fabrics and reagents, step by step, comfortably reproducible laboratory protocols, and worthy notes which spotlight pointers on troubleshooting and heading off identified pitfalls.
Comprehensive and straightforward to exploit, Lipoproteins and heart problems: tools and Protocols serves either newbies and specialists alike as a whole advisor for any researcher with an curiosity in lipoproteins and their major organic effects.