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By Herbert M. Richards

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It may be safely affirmed that whatever carbon dioxide was found came from the plants themselves and that these plants were entirely normal and healthy. The sunlight to which the material was exposed was as direct and as uninfluenced by interposed screens as was possible with the apparatus at hand. The method, previously referred to, of immersion in clear water cooled with was resorted to in order to prevent undue rise in temperature. It is realsome of the intensity of the sunlight is lost in transmission through the glass and the water and that the results do not give the entire possible For this latter purpose it would be necessary effect of exposure to the sun.

None of the experiments exposed for long periods in the dark at constant temperatures shows any indication that there is a persistence of periodicity in acidity beyond the immediate effect of the factors which caused it. Special experiments to reveal any derived periodicity did not in any case indicate diurnal oscillations other than those caused by illumination and temperature. Material of low acidity, when its original 32 ACIDITY AND GAS INTERCHANGE IN CACTI. placed in the dark, shows an increase of acid up to a certain point, which is Material of high followed by a slow decrease if the darkness is prolonged.

The first were with material sent from Tucson to New York, and the reactions, while not as marked as those carried on in the natural habitat of the plant, are worthy of record. This Various trials oxygen, in those in series of determinations is shown in table 17, series I. The material was exposed in an atmosphere of hydrogen, 97 per cent pure. 6 per cent, which is almost negligible. Analyses of the atmosphere of the end of the experiment showed no trace of oxygen, which must have disappeared almost at the outset.

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Acidity and gas interchange in cacti by Herbert M. Richards

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